Golnaz Taasoli; Farrokh Kafilzadeh; Darab Ghadimi; Juan Loor; Micheal Ballou
Volume 22, Issue 4 , December 2020, , Pages 529-536
Abstract
The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk ...
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The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk and 28 ± 6.6 kg of average milk production were used. All cows received a control diet containing low (34.3 percentage of dry matter) non fibrous carbohydrates content for the first 14 days and then were were switched to a diet containing high (40.7 percentage of dry matter) non fibrous carbohydrates content for one week. Blood samples were collected on day 0 (After 14 days of feeding control diet) and days 4 and 7 after feeding high NFC diet. Relative gene expression of seven genes (IL1-β, TNF-α, MNDA, CD74, TLR2, SELECTIN, TGF1-β) were measured. The results showed that gene expression of the pro-inflammatory cytokines (IL1-β and TNF-α) and anti-inflammatory proteins (MNDA, D74, TLR2, SELECTIN, TGF1-β) was down regulated during feeding of high NFC diet (p < 0.05). These data indicated that feeding a high non-fibrous carbohydrates diet caused inflammation and affected immunity system in lactating Jersey cows.
Asma Absalan; Golnaz Taasoli; Shahryar Kargar; Farshid Fatahnia; Zohre Kowsar; Alidad Boostani
Volume 21, Issue 1 , April 2019, , Pages 11-22
Abstract
This experiment was aimed to study the effect of multi enzyme and bacterial inoculant on chemical composition and fermentation parameters of barley silage. Robovio multi enzyme was added at three levels, 0, 500 and 1000 mg/kg of barley dry matter (DM) and Prosage bacterial inoculant (a mixture of Lactobacillus ...
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This experiment was aimed to study the effect of multi enzyme and bacterial inoculant on chemical composition and fermentation parameters of barley silage. Robovio multi enzyme was added at three levels, 0, 500 and 1000 mg/kg of barley dry matter (DM) and Prosage bacterial inoculant (a mixture of Lactobacillus buchneri,Lactobacillus plantarum, Enterococcus faecium and Propionibacterium acidophilus) was added at three levels, 0, 5 and 10 mg/kg of silage DM. Chemical composition, in vitro gas production, protozoa population and protein fractions of experimental silages were measured. Results showed that multi enzyme addition decreased NDF (P<0.05), pH, ash, B2 and C fractions (P<0.01) and increased A and B3 fractions (P<0.01) compared to the control. Barley silage containing 5 mg bacterial inoculant had greater DM, pH and B2 fraction (P<0.01) and lower C fraction compared to the control and silage containing 10 mg of bacterial inoculant. Protozoa population, N-ammonia concentration, estimated metabolizable energy, short chain fatty acids and in vitro organic matter digestibility were not affected (P>0.05). Based on these results use of bacterial and multi enzyme additives for the preparation of wilted barley silage is not recommended due to their costs.